In this study, we demonstrated that when Capto MMC ImpRes chromatography is conducted under weak partitioning mode, high throughput, good yield, and effective byproduct removal are simultaneously achieved.īispecific antibody (bsAb) Capto MMC ImpRes Light chain (LC)-missing byproduct Mixed-mode chromatography Weak partitioning mode WuXiBody.Ĭopyright © 2021 Elsevier Inc. 3 Dynamic binding capacity at 10 breakthrough by frontal analysis at a mobile phase. Multimodal cation exchanger with a different selectivity than traditional ion exchangers. The pressure given shall not be taken as the maximum pressure of the resin. It has a different selectivity, compared with traditional ion exchangers. However, the dynamic binding capacity (DBC) of Capto MMC ImpRes is relatively low under the selected condition, making the process less desirable for large-scale manufacturing. Overview Specifications Related Documents Order Table Product Support Capto MMC is a multimodal weak cation exchanger that is salt-tolerant. Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. We previously showed that Capto MMC ImpRes mixed-mode chromatography under bind-elute mode can effectively remove this LC-missing species. While for bsAbs based on other platforms removal of such byproduct can pose considerable challenge to the downstream team, in this case WuXiBody's unique design makes separation relatively straightforward. For purifying a WuXiBody-based symmetric bsAb, we identified a LC-missing species as a major byproduct. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. The medium combines high rigidity with high dynamic binding capacity (DBC) and. In this work, with two concrete cases (WuXiBodies with asymmetric and symmetric designs, respectively), we showed that Capto MMC ImpRes mixed-mode chromatography can effectively remove.
Its key feature is the replacement of one parental antibody's CH1/CL region with the T cell receptor (TCR) constant domain, a design that promotes cognate heavy chain (HC)-light chain (LC) pairing. Capto S is a chromatography medium based on the novel high flow agarose platform. it will be advantageous to identify the dynamic binding capacity (DBC) of the Protein. WuXiBody is a bispecific antibody (bsAb) platform developed by WuXi Biologics. An example of such a column is a Capto MMC, Capto MMC ImpRes (GE. working range 2-12 capacity >45 mg binding capacity (BSA/ml medium at 30 mS/cm) (Dynamic binding capacity at 10 breakthrough as measured at a residence time of 2 min, 300 cm/h in a Tricorn 5/100 column with 10-cm bed height in 50 mM Sodium acetate, pH 4.75, 250 mM NaCl.
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